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HBTU BP EP USP Pharma Grade arrives as a reagent-grade material essential to peptide synthesis in both laboratory and industrial pharmaceutical settings. The full name, O-Benzotriazole-N,N,N’,N’-tetramethyluronium hexafluorophosphate, identifies a compound specific in application and reliable in performance, prized for efficient coupling reactions. Source and purity reach strict criteria set out by BP (British Pharmacopoeia), EP (European Pharmacopoeia), and USP (United States Pharmacopeia), so the standards assure quality and reproducibility batch after batch. Recognized globally, HBTU holds its own HS Code for customs classification, which sits at 292529 for export and regulatory tracking purposes, letting import-export teams mark it clearly through supply chains.
Chemically, HBTU’s structure consists of a uronium core bonded to a benzotriazole moiety and balanced by the hexafluorophosphate counterion. The formula, C11H15N4OPF6, gives it both bulk and versatility. Its molecular weight hits about 380.23 g/mol, so calculations in reaction stoichiometry remain straightforward. Under the microscope, this molecule forms a solid, often showing up at the bench as off-white to cream flakes, sometimes powder, depending on the manufacturer. Rarely, the substance comes crystalline or pearled, since handling in synthesis calls for easy dissolution and transfer. Density clocks in at about 1.2 g/cm³, dense enough for efficient packing in storage but light enough to manage in standard laboratory vessels. HBTU dissolves well in DMF, DCM, and other polar aprotic solvents, which matters for process yield and shelf stability in peptide manufactories. Its stable nature at ambient temperature means long shelf-life, something both chemists and procurement teams appreciate.
In the jar, HBTU appears as a free-flowing solid, neither sticky nor hygroscopic under normal atmospheric conditions. This keeps spills easy to clean and ensures precise weighing—always a challenge with reagents that pick up water or clump. Flakes stand out as a popular particle form, with powder also common. Both forms handle well by spoon or spatula, and dissolve quickly during blending with organic media. HBTU carries the subtle odor characteristic of triazole compounds, but not overpowering like some other activating agents, which makes long hours in the lab less taxing. Technicians appreciate not having to deal with excess dust or static charges during transfer, a small but critical aspect of industrial usage.
Suppliers run HBTU through a suite of analytical tests before shipping, always crucial for applications where batch-to-batch reliability counts. Purity, by HPLC or titrimetric analysis, routinely exceeds 99 percent, securing confidence for regulated pharmaceutical environments. Every lot comes with GC-MS or NMR data, so users know what impurities, if any, hide inside. Solubility specs back up formulation decisions, and melting point typically falls in the 155–160°C range. Water content, by Karl Fischer titration, remains below 0.5 percent, preventing unwanted side reactions or decomposition in storage. Heavy metal and residual solvent levels sit well inside ICH Q3C guidelines, meaning the finished peptides pass stringent quality control down the line.
Research groups and drug production outfits alike reach for HBTU as a coupling agent during solid-phase peptide synthesis. Its consistent activation of carboxylic acids leads to high yields, and it leaves byproducts that remain easy to remove in downstream purification. Unlike some older carbodiimides, HBTU keeps racemization low, preserving the stereochemistry of delicate amino acid substrates. Its power as a reagent shortens process time, cuts down raw material consumption, and reduces operational costs over the lifetime of a campaign. I remember several projects where switching to HBTU tipped the scale from low double-digit yields to robust multi-gram runs, saving weeks in troubleshooting or resynthesis.
With its benefits, HBTU does carry certain health hazards. Handling the dry chemical always demands gloves, goggles, and a dust mask, as it can irritate eyes, skin, and mucous membranes on contact. Ingestion or inhalation, whether by accident or carelessness, brings the risk of harm, so maintaining best lab practices remains non-negotiable. Lab managers store HBTU in closed containers, away from light and moisture, not only for safety but, just as importantly, to preserve reactivity. Disposing of unused material or waste calls for compliance with local hazardous chemical regulations, since the hexafluorophosphate ion lifts it into a different category from basic organic waste streams. On large sites, containment systems and spill response plans stay ready to go, because a single gram on the loose creates cleanup headaches and potential for environmental contamination.
Production of HBTU depends on a few specialty raw materials, each tracked for purity and compliance. Benzotriazole, tetramethyluronium derivatives, and hexafluorophosphoric acid or salts form the backbone of synthesis. Manufacturers check incoming batches by certificate of analysis and reserve samples, keeping audit records in line with both cGMP guidelines and supply chain verification programs. Knowing where each precursor originates reduces the risk of contamination, substitution, or reaction failure—issues that can grind the most promising research to a halt. For pharma and biotech companies, documenting every step from raw material through to packed reagent stands as real evidence of due diligence, and it provides the traceability vital for clients, regulators, and end users.
Innovation in peptide chemistry still chases better yields and greener processes. Teams worldwide often study alternatives to hexafluorophosphate salts, looking to cut down hazardous waste and lower residual fluorine emissions. There’s also growing interest in biodegradable coupling agents or recycled solvent recovery from HBTU-driven reactions. Some new startups have focused on less toxic counterions or even glass-packed product forms to minimize airborne dust and accelerate dissolution. Safety data sheets, certificates of analysis, and validation packages grow thicker every year, reflecting a field where transparency, quality control, and environmental stewardship matter more than ever to everyone from bench scientists to regulatory inspectors. As precision medicine expands, HBTU and similar compounds play a frontline role, so investment in safer, more responsible handling and packaging will continue to define competitive manufacturers and trusted suppliers alike.
Chengguan District, Lanzhou, Gansu, China
